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oligo dt magnetic beads  (New England Biolabs)


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    Structured Review

    New England Biolabs oligo dt magnetic beads
    Oligo Dt Magnetic Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 979 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/oligo+dt+beads/Oligo+dT25+Mag+Beads/pmc13171292-311-8-12
    Average 98 stars, based on 979 article reviews
    oligo dt magnetic beads - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Isolation:

    Article Title: YTHDF2 suppresses the 2C-like state in mouse embryonic stem cells via the DUX-ZSCAN4 molecular circuit
    Article Snippet: Below are the antibodies utilized in the experiments: anti-YTHDF1 (Proteintech, 17479-1-AP), anti-YTHDF2 (Proteintech, 24744-1-AP), anti-YTHDF3 (Proteintech, 25537-1-AP), anti-ZSCAN4 (Abclonal, A10205), anti-MERVL-gag (Beyotime, AF0240), anti-DUX (HUABIO, ER1901–52), anti-CNOT1 (Proteintech, 14276-1-AP). .. Total RNA was extracted using Trizol reagent (Thermo Fisher Scientific), followed by mRNA isolation from the total RNA using oligo-dT beads (New England BioLabs, S1419S) to deplete rRNA. ..

    Article Title: DDX24 modulates angiogenesis by promoting CCR4-NOT complex-dependent mRNA decay
    Article Snippet: .. For the input control, RNA was isolated using RNAzol, treated with DNase I, and enriched for mRNA using Oligo-dT beads (NEB, E7490). ..

    Article Title: Single Nucleus MultiOmics and In Vivo Massively Parallel Reporter Assays Identify Functional Sex-Biased Enhancers Linked to Liver Metabolism and Disease
    Article Snippet: .. To extract transcribed reporter RNA, 120 μg of total liver RNA was isolated from 60-100 mg liver tissue from each mouse by TRIzol guanidinium thiocyanate-phenol-chloroform extraction followed by polyA selection using oligo(dT) beads (New England Biolabs, cat. #E7490L). .. The transcribed RNA was digested with Turbo DNase I (Ambion, cat. # AM2238) at 37°C for 30 min, reverse transcribed using primer ON8731 for STARR-seq RNA first strand synthesis and Superscript III first-strand synthesis supermix (Invitrogen, cat. # 18080-400) and treated with 10 μg RNase A per ml (Fermentas, cat. # EN0531) for 1 h at 37 ◦ C. The cDNA obtained was purified using 1.8x SPRI beads and then used as a PCR template in the nested PCR reaction.

    Article Title: DDX24 modulates angiogenesis by promoting CCR4-NOT complex-dependent mRNA decay.
    Article Snippet: .. For the input control, RNA was isolated using RNAzol, treated with DNase I, and enriched for mRNA using Oligo-dT beads (NEB, E7490). ..

    Article Title: Direct and transgenerational effects of tetracyclines on the microbiome, transcriptome, and male mating behavior of the sheep blowfly Lucilia cuprina
    Article Snippet: Illumina RNA library construction and sequencing were performed by the North Carolina State Genomic Sciences Laboratory (Raleigh, NC, USA). .. Samples were enriched for mRNA using oligo-dT beads provided in the NEBNExt Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, USA). cDNA libraries for Illumina sequencing were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB) and NEBNext Mulitplex Oligos for Illumina (NEB) using the manufacturer-specified protocol. .. Samples were selected for a final library size (adapters included) of 400 to 550 bp using sequential AMPure XP bead isolation (Beckman Coulter, USA).

    Control:

    Article Title: DDX24 modulates angiogenesis by promoting CCR4-NOT complex-dependent mRNA decay
    Article Snippet: .. For the input control, RNA was isolated using RNAzol, treated with DNase I, and enriched for mRNA using Oligo-dT beads (NEB, E7490). ..

    Article Title: DDX24 modulates angiogenesis by promoting CCR4-NOT complex-dependent mRNA decay.
    Article Snippet: .. For the input control, RNA was isolated using RNAzol, treated with DNase I, and enriched for mRNA using Oligo-dT beads (NEB, E7490). ..

    Extraction:

    Article Title: Single Nucleus MultiOmics and In Vivo Massively Parallel Reporter Assays Identify Functional Sex-Biased Enhancers Linked to Liver Metabolism and Disease
    Article Snippet: .. To extract transcribed reporter RNA, 120 μg of total liver RNA was isolated from 60-100 mg liver tissue from each mouse by TRIzol guanidinium thiocyanate-phenol-chloroform extraction followed by polyA selection using oligo(dT) beads (New England Biolabs, cat. #E7490L). .. The transcribed RNA was digested with Turbo DNase I (Ambion, cat. # AM2238) at 37°C for 30 min, reverse transcribed using primer ON8731 for STARR-seq RNA first strand synthesis and Superscript III first-strand synthesis supermix (Invitrogen, cat. # 18080-400) and treated with 10 μg RNase A per ml (Fermentas, cat. # EN0531) for 1 h at 37 ◦ C. The cDNA obtained was purified using 1.8x SPRI beads and then used as a PCR template in the nested PCR reaction.

    Selection:

    Article Title: Single Nucleus MultiOmics and In Vivo Massively Parallel Reporter Assays Identify Functional Sex-Biased Enhancers Linked to Liver Metabolism and Disease
    Article Snippet: .. To extract transcribed reporter RNA, 120 μg of total liver RNA was isolated from 60-100 mg liver tissue from each mouse by TRIzol guanidinium thiocyanate-phenol-chloroform extraction followed by polyA selection using oligo(dT) beads (New England Biolabs, cat. #E7490L). .. The transcribed RNA was digested with Turbo DNase I (Ambion, cat. # AM2238) at 37°C for 30 min, reverse transcribed using primer ON8731 for STARR-seq RNA first strand synthesis and Superscript III first-strand synthesis supermix (Invitrogen, cat. # 18080-400) and treated with 10 μg RNase A per ml (Fermentas, cat. # EN0531) for 1 h at 37 ◦ C. The cDNA obtained was purified using 1.8x SPRI beads and then used as a PCR template in the nested PCR reaction.

    Construct:

    Article Title: Direct and transgenerational effects of tetracyclines on the microbiome, transcriptome, and male mating behavior of the sheep blowfly Lucilia cuprina
    Article Snippet: Illumina RNA library construction and sequencing were performed by the North Carolina State Genomic Sciences Laboratory (Raleigh, NC, USA). .. Samples were enriched for mRNA using oligo-dT beads provided in the NEBNExt Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, USA). cDNA libraries for Illumina sequencing were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB) and NEBNext Mulitplex Oligos for Illumina (NEB) using the manufacturer-specified protocol. .. Samples were selected for a final library size (adapters included) of 400 to 550 bp using sequential AMPure XP bead isolation (Beckman Coulter, USA).



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